Comparative Analysis of Leguminous and Non-Leguminous Tree species in terms of Leaf Chemistry and Nutrient Cycling

Manisha Pragya, PURABI SAIKIA


The present study aimed to analyse the rhizospheric soils of leguminous (Dalbergia sissoo, and Tamarindus indica), and non-leguminous (Mangifera indica, and Ficus benghalensis) tree species and their leaf pigment concentration in a natural forest of Ranchi in pre-monsoon (February 2020), and post-monsoon (November 2019) seasons. Soil nutrient status was better during the post-monsoon season, while leaf pigment concentration was higher during the pre-monsoon season. Again, soil nutrient status was better in rhizospheric soils of legumes as compared to the non-legumes. SOC (%) was quite high in the rhizospheric soil of leguminous trees that ranged from 0.75-0.86 % during pre-monsoon, and 0.88-0.92% during post-monsoon seasons. The highest SOC (%) was found in rhizospheric soils of D. sissoo (0.92±0.04), lowest BD (1.08±0.11 gm cm-3) in rhizospheric soils of M. indica, while AN (kg ha-1), and TKN (%) both were highest in rhizospheric soils of T. indica (AN: 470.68±50.53, TKN: 0.42±0.09) during post-monsoon season. On the other hand, pigment concentration (mg gm-1) including chlorophyll a, b, total chl, and total carotenoids were highest in leaves of M. indica (Chl A: 1.15±0.04, Chl B: 0.82±0.01, total Chl: 1.71±0.04, and total carotenoids: 30.61±0.87) in pre-monsoon season. Rhizospheric soil nutrient status of legumes was better during both the pre-and post-monsoon seasons in comparison to non-legumes. Leguminous tree species are very important from an ecological point of view in terms of their nutrient recycling efficiency and photosynthetic ability. Therefore, it can be suggested for afforestation, and social forestry programs for sustainable ecological development as they can easily grow under natural conditions without any extra care.


Soil organic carbon, Total Kjeldahl nitrogen, Chlorophylls, Carotenoids, Legumes, Non-legumes


Al-Mujahidy, S.M.J., Hassan, M.M., Rahman, M.M., and Mamun-Or-Rashid, A.N.M. 2013. Study on measurement and statistical analysis of adherent soil chemical compositions of leguminous plants and their impact on nitrogen fixation. International Journal of Biosciences, 3, 112-119.

Allison, S.D., Nielsen, C., and Hughes, R.F. 2006. Elevated enzyme activities in soils under the invasive nitrogen-fixing tree Falcataria moluccana. Soil Biology and Biochemistry, 38, 1537-1544.

Allison, L. (1965). Organic carbon. Methods of soil analysis: part 2 chemical and microbiological properties, Agron Monographs, 9, 1367–1378.

Angus, J.F. and Van Herwaarden, A.F. 2001. Increasing water use and water use efficiency in dryland wheat. Agronomy Journal, 93(2), 290-298.

Arnon, D.I. 1937. Ammonium and nitrate nitrogen nutrition of barley at different seasons in relation to hydrogen-ion concentration, manganese, copper, and oxygen supply. Soil Science, 44(2), 91-122.

Bhattacharjee, R.B., Singh, A., and Mukhopadhyay, S.N. 2008. Use of nitrogen-fixing bacteria as biofertiliser for non-legumes: prospects and challenges. Applied Microbiology and Biotechnology, 80(2), 199-209.

Biederbeck, V.O., Janzen, H.H., Campbell, C.A., and Zentner, R.P. 1994. Labile soil organic matter as influenced by cropping practices in an arid environment. Soil Biology and Biochemistry, 26(12), 1647-1656.

Bonner, J. 1946. The role of organic matter, especially manure, in the nutrition of rice. Botanical Gazette, 108(2), 267-279.

Brown, S. and Lugo, A.E. 1990. Effects of forest clearing and succession on the carbon and nitrogen content of soil in Puerto Rico and US Virgin Islands. Plant Soil, 124, 53-64.

Chaer, G.M., Resende, A.S., Campello, E.F.C., de Faria, S.M., and Boddey, R.M. 2011. Nitrogen-fixing legume tree species for the reclamation of severely degraded lands inBrazil. Tree Physiology, 312, 139-149.

Chen, X.Y. and Mulder, J. 2007. Atmospheric deposition of nitrogen at five subtropical forested sites in South China. Science of the Total Environment, 378(3), 317-330.

Craswell, E.T. and Lefroy, R.D.B. 2001. The role and function of organic matter in tropical soils. In: Managing Organic Matter in Tropical Soils: Scope and Limitations, Springer, Dordrecht, pp. 61:7-18.

Crews, T.E. and Peoples, M.B. 2004. Legume versus fertilizer sources of nitrogen: ecological tradeoffs and human needs. Agriculture, Ecosystems & Environment, 102(3), 279-297.

Davidson, E.A., Carvalho, C.J.R., Vieira, I.C.G., Figueiredo, R.O., Moutinho, P., Ishida, R.Y., Santos, M.T.P., Guerrero, J.B., Kalif, K., and Saba, R.T. 2004. Nitrogen and phosphorus limitation of biomass growth in a tropical secondary forest. Ecological Applications, 14, 150-163.

Dixon, R. and Kahn, D. 2004. Genetic regulation of biological nitrogen fixation. Nature Reviews Microbiology, 2, 621-631.

Edwards, J.T.C., Cutler, D.R., Zimmermann, N.E., Geiser, L., and Moisen, G.G. 2006. Effects of sample survey design on the accuracy of classification tree models in species distribution models. Ecological Modelling, 199, 132-141.

Elmerich, C. and Newton, W.E. (eds.). 2007. Associative and endophytic nitrogen-fixing bacteria and cyanobacterial associations, Vol. 5, Dordrecht: Springer.

Feldpausch, T.R., Rondon, M.A., Fernandes, E.C., Riha, S.J., and Wandell, E. 2004. Carbon and nutrient accumulation in secondary forests regenerating on pastures in central Amazonia. Ecological Applications, 14, S164–S176.

Franche, C., Lindstrom, K., and Elmerich, C. 2009. Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant and Soil, 321(1-2), 35-59.

Francis, R. and Read, D.J. 1994. The contributions of mycorrhizal fungi to the determination of plant community structure. Plant and Soil, 159(1), 11-25.

Fyllas, N.M., Patino, S., Baker, T.R., Bielefeld, N.G., Martinelli, L.A., Quesada, C.A., and Santos, A. 2009. Basin-wide variations in foliar properties of Amazonian forest: phylogeny, soils and climate. Biogeosciences, 6, 2677-2708.

Gei, M.G. and Powers, J.S. 2013. Do legumes and non-legumes tree species affect soil properties in unmanaged forests and plantations in Costa Rican dry forests? Soil Biology and Biochemistry, 57, 264-272.

Ghosh, B.P. and Burris, R.H. 1950. Utilization of nitrogenous compounds by plants. Soil Science, 70(3), 187-204.

Giri, S., Shrivastava, D., Deshmukh, K., and Dubey, P. 2013. Effect of Air Pollution on Chlorophyll Content of Leaves. Current Agriculture Research Journal, 1(2), 93-98.

Graham, P.H. and Vance, C.P. 2003. Legumes: importance and constraints to greater use. Plant Physiology, 131(3), 872-877.

Gregorich, E.G. and Ellert, B.H. 1993. Light Fraction and Macroorganic. In: Soil Sampling and Methods of Analysis. Canadian Society of Soil Science, pp. 397.

Gregorich, E.G., Carter, M.R., Angers, D.A., Monreal, C., and Ellert, B. 1994. Towards a minimum data set to assess soil organic matter quality in agricultural soils. Canadian Journal of Soil Science, 74(4), 367-385.

Hoogmoed, M., Cunningham, S.C., Baker, P., Beringer, J., and Cavagnaro, T.R. 2014. N-fixing trees in restoration plantings: Effects on nitrogen supply and soil microbial communities. Soil Biology and Biochemistry, 77, 203–212.

Hughes, C.E. and Styles, B.T. 1989. The benefits and risks of woody legumes. Monographs in Systematic Botany from the Missouri Botanical Garden, 29(115), 505-531.

Iantchev, A., Mysore, K.S., and Ratet, P. 2013. Transformation of leguminous plants to study symbiotic interactions. International Journal of Developmental Biology, 57(6-7-8), 577-586.

Janzen, H.H., Campbell, C.A., Brandt, S.A., Lafond, G.P., and Townley‐Smith, L. 1992. Light‐fraction organic matter in soils from long‐term crop rotations. Soil Science Society of America Journal, 56(6), 1799-1806.

Jordan, C.F. 1985. Nutrient cycling in tropical forest ecosystems. John Wiley & Sons, Chichester, pp. 190.

Kjeldahl, J.G.C.T. 1883. A new method for the estimation of nitrogen in organic Compounds. Z. Anal. Chem, 22(1), 366–382.

Kumar, V., Pathak, D.V., Dudeja, S.S., Saini, R., Giri, R., Narula, S., and Anand, R.C. 2013. Legume nodule endophytes are more diverse than endophytes from roots of legumes or non-legumes in soils of Haryana, India. J. Microbiol. Biotech. Res, 3(3), 83-92.

Lefroy, E.C., Flugge, F., Avery, A., and Hume, I. 2006. Potential of current perennial plant-based farming systems to deliver salinity management outcomes and improve prospects for native biodiversity: a review. Australian Journal of Experimental Agriculture, 45(11), 1357-1367.

Lewis, G., Schrire, B., Mackinder, B., and Lock, M. 2005. Legumes of the World. Richmond, U.K. Royal Botanic Gardens, Kew.

Li, Y., He, N., Hou, J., Xu, L., Liu, C., Zhang, J., Wang, Q., Zhang, X., and Wu, X. 2018. Factors Influencing Leaf Chlorophyll Content in Natural Forests at the Biome Scale. Frontiers in Ecology and Evolution, 6, 64.

Maroti, G. and Kondorosi, E. 2014. Nitrogen-fixing Rhizobium-legume symbiosis: are polyploidy and host peptide-governed symbiont differentiation general principles of endosymbiosis? Frontiers in Microbiology, 5, 1-6.

NRC (National Research Council) 1981. Surface Mining Soil, Coal, and Society. National Academy Press: Washington, DC.

Palta, J.P. 1990. Leaf chlorophyll content. Remote Sensing Reviews, 5(1), 207-213.

Pennington, R.T., Prado, D.E., and Pendry, C.A. 2000. Neotropical seasonally dry forests and Quaternary vegetation changes. Journal of Biogeography, 27, 261-273.

Peoples, M.B., Brockwell, J., Herridge, D.F., Rochester, I.J., Alves, B.J.R., Urquiaga, S., and Sampet, C. 2009. The contributions of nitrogen-fixing crop legumes to the productivity of agricultural systems. Symbiosis, 48(1-3), 1-17.

Powers, J.S., and Tiffin, P. 2010. Plant functional type classifications in tropical dry forests in Costa Rica: leaf habit versus taxonomic approaches. Functional Ecology, 24(4), 927-936.

Prado, D.E. 2000. Seasonally dry forests of tropical South America: From forgotten ecosystems to a new phytogeographic unit. Edinburgh Journal of Botany, 57, 437-461.

Prado, D.E. and Gibbs, P.E. 1993. Patterns of species distributions in the dry seasonal forests of South America. Annals of the Missouri Botanical Garden, 80, 902-927.

Russell, R.S. 1977. Plant root systems. The soil environment. McGraw-Hill, London, pp. 143-168.

Saikia, P., Nag, A., Anurag, S., Chatterjee, S., and Khan, M.L. 2020. Tropical Legumes: Status, Distribution, Biology, and Importance. In: The Plant Family Fabaceae, Hasanuzzaman M., Araújo S., and Gill, S.S. (eds.), Chapter 2, Springer Nature Singapore Pte Ltd., pp. 27-41.

Siddique, I., Engel, V.L., Parrotta, J.A., Lamb, D., Nardoto, G.B., Ometto, J.P.H.B., Martinelli, L.A., and Schmidt, S. 2008. Dominance of legume trees alters nutrient relations in mixed species forest restoration plantings within seven years. Biogeochemistry, 88, 89-101.

Singh, A. 2015. Nitrogen and phosphorus resorption efficiency in some leguminous and non-leguminous tropical tree species planted on coal mines spoil in a tropical dry environment. Ambit J Educ Res Rev, 1, 1-7.

Six, J., Feller, C., Denef, K., Ogle, S.M., Moraes-Sa, J.C., and Albrecht, A. 2002. Soil organic matter, biota and aggregation in temperate and tropical soils: effects of no-tillage. Agronomie, 22, 755-775.

Sprent, J. I. 2009. Legume nodulation: a global perspective. John Wiley and Sons.

Sprent, J.I. and Parsons, R. 2000. Nitrogen fixation in legume and non-legume trees. Field Crops Research, 65(2-3), 183-196.

Steidinger, B.S., Crowther, T.W., Liang, J., et al. 2019. Climatic control of decomposition drives the global biogeography of forest tree symbioses. Nature, 569, 404-408

Swift, M.J., Heal, O.W., and Anderson, J.M. 1979. Decomposition in terrestrial ecosystems. Black- well Scientific Publications, Oxford, pp. 372.

Taylor, B.N. and Ostrowsky, L.R. 2018. Nitrogen-fixing and non-fixing trees differ in leaf chemistry and defence but not herbivory in a lowland Costa Rican rain forest. Journal of Tropical Ecology, 35(6), 270-279.

Tsutsumi, T. 1987a. The nitrogen cycle in a forest. Memoirs of College Agriculture, Kyoto University, 130, 1-16.

Tsutsumi, T. 1987b. Matter cycling in forest ecosystems. Tokyo University Press, Tokyo (in Japanese), pp. 124.

van Kessel, C. and Hartley, C. 2000. Agricultural management of grain legumes: has it led to an increase in nitrogen fixation? Field Crops Research, 65(2-3), 165-181.

van Kessel, C., Farrell, R.E., Roskoski, J.P., and Keane, K.M. 1994. Recycling of the naturally occurring N in an established stand of Leucaena leucocephala. Soil Biol. Biochem, 26, 757-762.

Venterink, H.O. 2011. Legumes have a higher root phosphatase activity than other forbs, particularly under low inorganic P and N supply. Plant and Soil, 347(1-2), 137-146.

Walkley, A. and Black, I.A. 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science, 37(1), 29–38.

Xia, J.Y. and Wan, S.Q. 2008. Global response patterns of terrestrial plant species to nitrogen enrichment. New Phytology, 179, 428-439.

Yamakura, T. and Sahunalu, P. 1990. Soil carbon/nitrogen ratio as a site quality index for some South-east Asian forests. Journal of Tropical Ecology, 6, 371-378.

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