International Journal of Ecology and Environmental Sciences
BIODISESEL PRODUCTION OF Microseira woelli ISOLATED FROM FRESH WATER PONDS
Abstract
Algal biofuel or algal oil is an alternative to liquid fossil fuels that uses algae as the source of energy and they are considered as a replacement for biofuel sources such as corn and sugarcane. This study presents the isolation of algal strain from fresh water ponds in Pudukkottai District. The algal strain was confirmed as Microseira wollei after PCR amplification and sequencing of the 16s rRNA gene of the DNA isolated from the species. The structure of the strain was observed using a Trinocular microscope. The phylogenetic tree analysis was carried out to find the evolutionary relationship of the organism. Biodiesel was produced from Microseira wollei by lipid extraction and allowed for trans-esterification. The biodiesel production of was Microseira wollei was confirmed by GC-MS analysis which showed high quantity of methyl ester compounds.
Keywords: Microseira woelli, biodiesel, 16s rRNA gene, trans-esterification, GC-MS
Keywords
References
Anto, S. Mukherjee, S.S. Muthappa, T. Mathimani, R. Deviram, G. Kumar, S.S. Verma, T.N. and Pugazhendhi, A. 2020. Algae as green energy reserve: Technological outlook on biofuel production. Chemosphere 242 12: 125079
_____________________________________________
Bruno, L. di Pippo, F. Antonaroli, S. Gismondi, A. Valentini, C. and Albertans, P. 2012. Characterization of biofilm-forming cyanobacteria for biomass and lipid production. Journal of Applied Microbiology 113:1052–1064.
___________________________________________
Chinnasamy, S. Bhatnagar, A. Hunt, R.W. Das, K.C. 2010. Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel application. Bioresource Technology 101:3097-3105
Falcon, L.I. Magallon, S. and Castillo, A. 2010. Dating the cyanobacterial ancestor of the chloroplast. ISME Journal 4:777–783.
________________________________________________
Fischer, W.W. Hemp, J. and Johnson, J.E. 2016. Evolution of oxygenic photosynthesis. Annual Review of Earth Planet Science 44: 647–683.
_________________________________________
Glithero, N.J. Wilson, P. and Ramsden, S.J. 2015. Optimal combinable and dedicated energy crop scenarios for marginal land. Applied Energy 147: 82–91.
___________________________________________________
Gnansounou, E. 2010. Production and use of lignocellulosic bioethanol in Europe: current situation and perspectives. Bioresource Technology 101: 4842–4850.
_____________________________________________________
Henry, T. Iwen, P. C. and Hinrichs, S. H. 2000. Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. Journal of clinical microbiology 38: 1510-1515.
__________________________________________________
Hossain, M. F. Ratnayake, R. R. Mahbub, S. Kumara, K. W. and Magana-Arachchi, D. N. 2020. Identification and culturing of cyanobacteria isolated from freshwater bodies of Sri Lanka for biodiesel production. Saudi Journal of Biological Sciences.12: 100-108.
__________________________________________________
Jensen, S. K. 2008. Improved Bligh and Dyer extraction procedure. Lipid Technology 20: 280-281.
Jorgensen, H. van Hecke, J. Zhang, H. Malik, P.L. Filby, C. and Schjoerring, J.K. 2018. Wheat as a dual crop for biorefining: straw quality parameters and their interactions with nitrogen supply in modern elite cultivars. GCB Bioenergy 11:400–415.
______________________________________________________
Kadam, K.L. Forrest, L.H. and Jacobson, W.A. 2000. Rice straw is a lignocellulosic resource: collection, processing, transportation, and environmental aspects. Biomass Bioenergy 18:369–389.
__________________________________________
Kasting, J.F. 2013. What caused the rise of atmospheric O2? Chemical Geology 362: 13–25.
Kim, S. and Dale, B.E. 2004. Global potential bioethanol production from wasted crops and crop residues. Biomass Bioenergy 26: 361–375.
______________________________________________
Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16: 111-120.
_________________________________________________
Klutz, I. Wicke, B. Leemans, R. and Faaij, A. 2017. Sustainability constraints in determining European bioenergy potential: a review of existing studies and steps forward. Renewable and Sustainable Energy Reviews 69: 719–734.
____________________________________________________
Koley, S. Khadase, M.S. Mathimani, T. Raheman, H. and Mallick, N. 2018. Catalytic and non-catalytic hydrothermal processing of Scenedesmus obliquus biomass for bio crude production – A sustainable energy perspective, Energy Conversvation 163:212-217.
_______________________________________________________
Kumar, S. Stecher, G. and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology and evolution 33: 1870-1874.
________________________________________________________
Mathimani, T. and Mallick, N. 2019. A review on the hydrothermal processing of microalgal biomass to bio-oil - Knowledge gaps and recent advances. Journal of cleaner production 217:69-84,
____________________________________________________
Mathimani, T. and Mallick, N. 2018. A comprehensive review on harvesting of microalgae for biodiesel - Key challenges and future directions, Renewable and Sustainable Energy Review 91:1103-1120
_______________________________________________________
Mazard S. Penesyan, A. Ostrowski, M. Paulsen, I.T. and Egan, S. 2016. Tiny Microbes with a Big Impact: The Role of Cyanobacteria and Their Metabolites in Shaping Our Future. Marine drugs 14: 97-100.
Murata, N. Wada, H. and Gombos, Z. 1993. Modes of fatty acid desaturation in cyanobacteria. Plant Cell Physiology 33:933–941.
___________________________________________________________
Nilsson, D. 2000. Dynamic simulation of straw harvesting systems: influence of climatic, geographical, and biological factors on performance and costs. Journal of Agricultural Engineering and Research 76: 27–36.
_________________________________________________
Panoutsou, C. Perakis, C. Elbersen, B. Zheliezna, T. and Staritsky, I. 2017. Assessing potentials for agricultural residues and Modeling and optimization of biomass supply chains. Academic Press 12: 169–197.
_____________________________________________________
Passoth, V. 2014. Molecular mechanisms in yeast carbon metabolism: bioethanol and other biofuels. Springer 1: 217–259.
______________________________________________________
Passoth, V. Tabassum, M.R. Nair, H.A. Olstorpe, M. Tiukova, I. and Stahlberg, J. 2013. Enhanced ethanol production from wheat straw by integrated storage and pre-treatment (ISP) Enzyme and Microbial Technology 52: 105–110.
____________________________________________________
Peng, X. and Chen, Y. 2008. Single cell oil production in solid-state fermentation by Microsphaeropsis sp. from steam-exploded wheat straw mixed with wheat bran. Bioresource Technology 99:3885-3889
________________________________________________________
Perumal, G.M. and Anand, N. Manual of Fresh water Algae of TamilNadu. Centre for Advanced studies in Botany, University of Madras, Guindy campus, Chennai-25. 116 pages
___________________________________________________
Rittmann, B.E. 2008. Opportunities for renewable bioenergy using microorganisms. Biotechnology and Bioengineering 2: 203–212.
___________________________________________________
Saravanan, A.P. Mathimani, T. Deviram, G. Rajendran, K. and Pugazhendhi, A. 2018. Biofuel policy in India: A review of policy barriers in sustainable marketing of biofuel. Journal of cleaner production 193:734-747.
____________________________________________
Schenk, P.M. ThomasHall, S.R. Stephens, E. Marx, U.C. Mussgnug, J.H. and Posten, C. 2008. Second generation biofuels: high-efficiency microalgae for biodiesel production. Bioenergy Resource 1: 20-43
Schirrmeister, B.E. Sanchez-Baracaldo, P. and Wacey, D. 2016. Cyanobacterial evolution during the Precambrian. International Journal of Astrobiology 15:187–204.
___________________________________________________
Sims, R.E.H. Mabee, W. Saddler, J.N. and Taylor, M. 2010. An overview of second-generation biofuel technologies. Bioresource Technology 101: 1570–1580.
_______________________________________________
Sneath P.H.A. and Sokal R.R. 1973. Numerical Taxonomy. Freeman, San Francisco.
_____________________________________
Talebnia, F. Karakashev, D. and Angelidaki, I. 2010. Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis, and fermentation. Bioresource Technology 101: 4744–4753.
________________________________________________________
Tamura, K. Nei, M. and Kumar, S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035.
___________________________________________________
Tishler, Y. Samach, A. Rogachev, I. Elbaum, R. and Levy, A.A. 2015. Analysis of wheat straw biodiversity for use as a feedstock for biofuel production. BioEnergy Research 8:1831–1839.
________________________________________________
Townsend, T.J. Sparkes, D.L. and Wilson, P. 2017. Food and bioenergy: reviewing the potential of dual-purpose wheat crops. GCB Bioenergy 9: 525–540.
___________________________________________________
Valentine, J. Clifton-Brown, J. Hastings, A. Robson, P. Allison, G. and Smith, P. 2012. Food vs. fuel: the use of land for lignocellulosic ‘next generation’ energy crops that minimize competition with primary food production. GCB Bioenergy 4:1–19.
__________________________________________________
Yang, J. Xu, M. Zhang, M. Hu, M. and Sommerfeld M. 2011. Life-cycle analysis on biodiesel production from microalgae: Water footprint and nutrients balance. Bioresource Technology 102:159-165
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.
COPYRIGHT of this Journal vests fully with the National Instional Institute of Ecology. Any commercial use of the content on this site in any form is legally prohibited.