International Journal of Ecology and Environmental Sciences
Diazotrophic endophytic bacterium Alcaligenes sp. KA31 and its role in promoting the growth of wheat (Triticum aestivum L.) under saline conditions
Abstract
Intensification of food production with an increasing world population, to achieve food security has become one of the major agricultural challenges for a sustainable future. Salinity is the second most abundant threat after drought which causes disturbance in the symbiotic performance of plants and increases their susceptibility to detrimental soil-borne pathogens. In the present study, endophytic diazotroph KA31 isolated from roots of wheat plant was characterized and identified as Alcaligenes faecalis. The bacterial endophytic isolate produced indole acetic acid (IAA), and siderophore along with the potential to solubilize phosphorous (P). KA31 was also able to fix nitrogen (N) as evaluated through acetylene reduction assay and nifH-PCR assay. Recolonization ability of A. faecalis in the wheat seedlings was studied under laboratory conditions and it was demonstrated that KA31 was able to establish an endophytic association with the host plant. Furthermore, in the pot experimental study, A. faecalis KA31 significantly improved the growth parameters of wheat plants under saline conditions as compared to untreated control plants. Presumably this is the first study that reports A. faecalis as salt tolerant diazotrophic plant growth promoting endophyte (PGPE) and overall the work depicts that novel bioformulations developed using them can be effective for wheat crops grown in saline soils.
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References
Afzal I, Shinwari ZK, Sikandar S, Shahzad S (2019) Plant beneficial endophytic bacteria: mechanisms, diversity, host range and genetic determinants. Microbiol Res 221: 36-49
Ahmad F, Ahmad I, Khan MS (2008) Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 163(2): 173–181
Akhdiya A, Wahyudi AT, Munif A, Darusman LK (2014) Characterization of an endophytic bacterium G062 isolate with beneficial traits. Hayati J Biosci 21(4): 187-196
Akintokun AK, Ezaka E, Akintokun PO, Shittu OB, Taiwo LB (2019) Isolation, Screening and Response of maize to plant growth promoting rhizobacteria inoculants. Scientia Agriculturae Bohemica 50: 181-190
Altschul FS, Warren G, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403–410
Anjum N, Chandra R (2015) Endophytic bacteria: optimizaton of isolation procedure from various medicinal plants and their preliminary characterization. Asian J Pharm Clin Res 8: 233–238
Antony CP, Kumaresan D, Ferrando L, Boden R, Moussard H et al. (2010) Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact. ISME J 4: 1470-1480
Arora NK, Fatima T, Mishra J, Mishra I, Verma S et al. (2020) Halo-tolerant plant growth promoting rhizobacteria for improving productivity and remediation of saline soils. J Adv Res 26: 69-82
Arora NK, Verma M (2017) Modified microplate method for rapid and efcient estimation of siderophore produced by bacteria. 3 Biotech 7(381):1–9
Ashraf M, Ozturk M, Athar HR (2009) Salinity and Water Stress: Improving Crop Efficiency. Springer, Dordrecht, Ed. 1, pp. 244. 10.1007/978-1-4020-9065-3.
Babar M, Rasul S, Aslam K, Abbas R, Manzoor I, Hanif MK, Naqqash T (2021) Mining of halo-tolerant plant growth promoting rhizobacteria and their impact on wheat (Triticum aestivum L.) under saline conditions. J King Saud Univ Sci 33(3): 101372
Bharti N, Pandey SS, Barnawal D, Patel VK, Kalra A (2016) Plant growth promoting rhizobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress. Sci Rep 6:34768
Bokhari A, Essack M, Lafi FF, Andres-Barrao C, Jalal R et al. (2019) Bioprospecting desert plant Bacillus endophytic strains for their potential to enhance plant stress tolerance. Sci Rep 9: 18154
de Souza RS, Okura VK, Armanhi J, Jorrín B, Lozano N, Silva M et al. (2016) Unlocking the bacterial and fungal communities assemblages of sugarcane microbiome. Sci Rep 6: 28774
Debouba M, Gouia H, Suzuki A, Ghorbel M (2007) NaCl stress effects on enzymes involved in nitrogen assimilation pathway in tomato “Lycopersicon esculentum” seedlings. J Plant Physiol 163: 1247-58
Díaz Herrera S, Grossi C, Zawoznik M, Groppa MD (2016) Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum. Microbiol Res 186: 37–43
Egamberdieva D, Wirth S, Behrendt U, Ahmad P, Berg G (2017) Antimicrobial activity of medicinal plants correlates with the proportion of antagonistic endophytes. Front Microb 8: 199
Elbeltagy A, Nishioka K, Sato T, Suzuki H, Ye B et al. (2001) Endophytic colonization and in planta nitrogen fixation by a Herbaspirillum sp. Isolated from wild rice species. Appl Environ Microbiol 67: 5285–5293
Elkoca E, Turan M, Donmez MF (2010) Effects of single, dual and triple inoculations with Bacillus subtilis, Bacillus megaterium and Rhizobium leguminosarum bv. phaseoli on nodulation, nutrient uptake, yield and yield parameters of common bean (Phaseolus vulgaris L. cv. ‘elkoca-05'). J Plant Nutr 33 (14): 2104–2119
Fasim F, Ahmed N, Parsons R, Gadd GM (2002) Solubilization of zinc salts by a bacterium isolated from the air environment of a tannery. FEMS Microbiol Lett 213:1–6
Fatima T, Arora NK (2021) Pseudomonas entomophila PE3 and its exopolysaccharides as biostimulants for enhancing growth, yield and tolerance responses of sunflower under saline conditions. Microbiol Res 244: 126671
Fatima T, Mishra I, Verma R, Arora NK (2020) Mechanisms of halotolerant plant growth promoting Alcaligenes sp. involved in salt tolerance and enhancement of the growth of rice under salinity stress. 3 Biotech 10: 361
Fróna D, Szenderák J, Harangi-Rákos M (2019) The Challenge of Feeding the World. Sustainability 11(20): e5816
Gaiero JR, McCall CA, Thompson KA, Day NA, Best AS, Dunfield KE (2013) Inside the root microbiome: bacterial root endophytes and plant growth promotion. Am J Bot 100: 1738-1750
Gamalero E, Lingua G, Caprì FG, Fusconi A, Berta G et al. (2004) Colonization pattern of primary tomato roots by Pseudomonas fluorescens A6RI characterized by dilution plating, flow cytometry, fluorescence, confocal and scanning electron microscopy. FEMS Microbiol Ecol 48: 7987
Garrity G (2005) The proteobacteria, Part B the gammaproteobacteria. In: Garrity G, Brenner DJ, Krieg NR, Staley JR (eds) Bergey’s manual of systematic bacteriology, vol 2. Springer, New York, pp 323–337
Gourion B, Berrabah F, Ratet P, Stacey G (2015) Rhizobium-legume symbioses: the crucial role of plant immunity. Trends Plant Sci 20: 186–194
Hallmann J, Berg G (2006) Spectrum and Population Dynamics of Bacterial Root Endophytes. In: Schulz BJE, Boyle CJC, Sieber TN (eds) Microbial Root Endophytes. Soil Biology vol 9 Springer, Berlin, Heidelberg
Hemida KA, Reyad AMM (2018) Improvement salt tolerance of safflower plants by endophytic bacteria. J Hortic Plant Res 5:38–56
Hernawati H, Wiyono S, Santoso S (2011) Leaf endophytic fungi of chili (Capsicum annuum) and their role in the protection against Aphis gossypii (Homoptera: Aphididae). Biodiversitas J Biol Divers 12 (4): 187−191
Igrejas G, Branlard G (2020) The importance of wheat. In: Igrejas G, Ikeda TM, Guzmán C Wheat quality for improving processing and human health. Springer International Publishing pp. 557
Iniguez AL, Dong Y, Triplett EW (2004) Nitrogen fixation in wheat provided by Klebsiella pneumoniae 342. Mol Plant Microbe Interact 17: 1078–1085
Jackson ML (1973) Soil chemical analysis. Prentice Hall of India Pvt. Ltd., New Delhi, pp 38–56
Jaemsaeng R, Jantasuriyarat C, Thamchaipenet A (2018) Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105. Sci Rep 8: 1950
Jha Y (2019) Endophytic bacteria mediated anti-autophagy and induced catalase, β-1,3-glucanases gene in paddy after infection with pathogen Pyricularia grisea. Indian Phytopathol 72: 99–106
Kandel SL, Firrincieli A, Joubert PM, Okubara PA, Leston ND et al. (2017) An in vitro study of bio-control and plant growth promotion potential of Salicaceae endophytes. Front Microbiol 8:386
Khan Z, Doty SL (2009) Characterization of bacterial endophytes of sweet potato plants. Plant Soil 322(1): 197-207
Khare E, Mishra J, Arora NK (2018) Multifaceted Interactions Between Endophytes and Plant: Developments and Prospects. Front Microbiol 9: 2732
Khare E, Singh S, Maheshwari DK, Arora NK (2011) Suppression of charcoal rot of chickpea by fluorescent Pseudomonas under saline stress condition. Curr Microbiol 62:1548–1553
Kifle MH, Laing MD (2016) Effects of selected diazotrophs on maize growth. Front Plant Sci 7:1429
Kiyani T, Khan SA, Noureen N, Yasmin T, Zakria M et al. (2019) Comparison of culturable endophytic bacterial community of stripe rust resistant and susceptible wheat cultivars. Int Microbiol 22:191–201
Knoth JL, Kim SH, Ettl GJ, Doty SL (2012) Effects of cross host species inoculation of nitrogen-fixing endophytes on growth and leaf physiology of maize. GCB Bioenergy 5:408–418
Kruasuwan W and Thamchaipenet A (2018) 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing endophytic diazotrophic Enterobacter sp. EN-21 modulates salt–stress response in sugarcane. J Plant Growth Regul 37(3): 849-858
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549
Lebeis SL, Paredes SH, Lundberg DS, Breakfield, N, Gehring J et al. (2015) Salicylic acid modulates colonization of the root microbiome by specific bacterial taxa. Science 349: 860–864
Liu XJA, van Groenigen KJ, Dijkstra P, Hungate BA (2017) Increased plant uptake of native soil nitrogen following fertilizer addition–not a priming effect?. Appl Soil Ecol 114: 105-110
Mittler R (2006) Abiotic stress, the field environment and stress combination. Trends Plant Sci 11(1): 15-19
Momose A, Ohtake N, Sueyoshi K, Sato T, Nakanishi Y et al. (2009) Nitrogen fixation and translocation in young sugarcane (Saccharum officinarum L.) plants associated with endophytic nitrogen-fixing bacteria. Microbes Environ 24: 224-230
Okon Y, Albrecht SL, Burris RH (1977) Methods for growing Spirillum lipoferum and for counting it in pure culture and in association with plants. Appl Environ Microbiol 33: 85-88
Otlewska A, Migliore M, Dybka-Stępień K, Manfredini A, Struszczyk-Świta K et al. (2020) When Salt Meddles Between Plant, Soil, and Microorganisms. Front Plant Sci 11: 553087
Panigrahi S, Mohanty S, Rath CC (2020) Characterization of endophytic bacteria Enterobacter cloacae MG00145 isolated from Ocimum sanctum with Indole Acetic Acid (IAA) production and plant growth promoting capabilities against selected crops. S Afr J Bot 134: 17-26
Petrini O (1991) Fungal Endophytes of Tree Leaves. In: Andrews J.H., Hirano S.S. (eds) Microbial Ecology of Leaves. Brock/Springer Series in Contemporary Bioscience. Springer, New York, NY. Doi: 10.1007/978-1-4612-3168-4_9
Pikovskaya RI (1948) Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiology 17:362–370
Qiao H, Huang L, Kang Z (2006) Endophytic bacteria isolated from wheat and their antifungal activities to soil-borne disease pathogens. Chin J Appl Ecol 17(4): 690–694
Rajawat MVS, Singh S, Tyagi SP, Saxena AK (2016) A modified plate assay for rapid screening of potassium-solubilizing Bacteria. Pedosphere 26: 768–773
Rashid S, Charles TC, Glick BR (2012) Isolation and characterization of new plant growth-promoting bacterial endophytes. Appl Soil Ecol 61: 217-224
Ray S, Singh V, Singh S, Sarma BK, Singh HB (2016) Biochemical and histochemical analyses revealing endophytic Alcaligenes faecalis mediated suppression of oxidative stress in Abelmoschus esculentus challenged with Sclerotium rolfsii. Plant Physiol Biochem, 109: 430-441
Rosado AS, Duarte GF, Seldin L, Elsas JD van, Van Elsas JD (1998) Genetic Diversity of nifH Gene Sequences in Paenibacillus azotofixans Strains and Soil Samples Analyzed by Denaturing Gradient Gel Electrophoresis of PCR-Amplified Gene Fragments. Appl Environ Microbiol 64(8): 2770–2779
Rosenblueth M, Martínez-Romero E (2006) Bacterial endophytes and their interactions with hosts. Mol Plant Microbe Interact 19:827–837
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Santi C, Bogusz D, Franche C (2013) Biological nitrogen fixation in non-legume plants. Ann Bot 111: 743–767
Sayyed RZ, Gangurde NS, Patel PR, Joshi SA, Chin-Cholker SB (2010) Siderophore production by Alcaligenes faecalis and its application for growth promotion in Arachis hypogaea. Indian J Biotechnol 9: 302-307
Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56
Shewry PR and Hey SJ (2015) The contribution of wheat to human diet and health. Food Ener Secur 4(1): 178-202
Souza R, Beneduzi A, Ambrosini A, Costa PB, Meyer J et al. (2013). The effect of plant growth-promoting rhizobacteria on the growth of rice (Oryza sativa L.) cropped in southern Brazilian fields. Plant Soil 366:585-603
Terakado-Tonooka J, Owaki Y, Yamakawa H, Tanaka F, Yoneyama T et al. (2008) Expressed nifH genes of endophytic bacteria detected in field-grown sweet potatoes (Ipomoea batatas L.). Microbes Environ 23: 89–93
Tewari S, Arora NK (2016) Fluorescent Pseudomonas sp. PF17 as an efficient plant growth regulator and biocontrol agent for sunflower crop under saline conditions. Symbiosis 1(3):99–108
Ueda T, Suga Y, Yahiro N, Matsuguchi T (1995) Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences. J Bacteriol 177: 1414–1417
Vandana UK, Singha B, Gulzar ABM, Mazumder PB (2020) Molecular mechanisms in plant growth promoting bacteria (PGPR) to resist environmental stress in plants. In: Molecular Aspects of Plant Beneficial Microbes in Agriculture, pp 221-233
Vigani G, Rolli E, Marasco R, Dell'Orto M, Michoud G, Soussi A, Daffonchio D (2019) Root bacterial endophytes confer drought resistance and enhance expression and activity of a vacuolar H+‐pumping pyrophosphatase in pepper plants. Environ Microbiol 21(9): 3212-3228
Weller DM, Cook RJ (1983) Suppression of take-all of wheat by seed treatment with fluorescent pseudomonads, Phytopathology 73: 463
Xing YM, Chen J, Cui JL, Chen XM, Guo SX (2011) Antimicrobial activity and biodiversity of endophytic fungi in Dendrobium devonianum and Dendrobium thyrsiflorum from Vietman. Curr Microbiol 62(4): 1218-1224
Yadav A and Yadav K (2017) Exploring the potential of endophytes in agriculture: A minireview. Adv Plants Agric Res 6(4): 102-106
Yan Y, Kuramae EE, Klinkhamer PG, van Veen JA (2015) Revisiting the dilution procedure used to manipulate microbial biodiversity in terrestrial systems. Appl Environ Microbiol 81: 4246–4252
Zehr JP, Jenkins BD, Short SM, Steward GF (2003) Nitrogenase gene diversity and microbial community structure: a cross-system comparison. Environ Microbiol 5 (7): 539-554
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