International Journal of Ecology and Environmental Sciences
Production and partial characterization of alkaline chitinase from bacterial strain Aeromonas dhankensis PZ6 isolated from Tithal coastline of Valsad, Gujarat, India
Abstract
Chitin is the most widespread renewable polymer present in ocean and land. It is a crucial source of carbon and nitrogen for many marine organisms. The goal of this research was to identify and enhance the production of chitinase from bacterial isolates from the Tithal coastline in Valsad, Gujarat, India. A total 100 different soil samples were screened using colloidal chitin agar medium for chitinolytic activity. Based on maximum zone of chitin hydrolysis PZ6 isolate was selected for submerged fermentation (SmF). Optimization of chitinase production in reference to production time, carbon source, nitrogen source, inoculum size, pH, temperature and metal ions were investigated. Highest chitinase production observed with 0.8 % (w/v) colloidal chitin;8 pH 35°C temperature under shaking conditions after 24 hours. The most favored nitrogen and carbon sources were yeast extract and glucose. The growth rate increased when medium was supplemented with Co+2 metal ions. The molecular weight of purified chitinase as determined by SDS-PAGE technique was 60 kD. The results demonstrated the significance of PZ6 isolate as a potent biocontrol agent for plant pathogenic fungi and as a tool for sustainable conversion of natural abundant chitinous waste into various value-added products.
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References
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