Non-Edible Oil Cakes as Organic Amendment for the Growth of Cenchrus setigerus and its Effect on Naturally Occurring Azospirillum in the Rhizosphere

Kalpana Arora, Abhishek Sharma, Garima Tiwari, Kanika khanna, Vaibhav Yadav, Satyawati Sharma


The present study was carried out to investigate the integrated effect of non-edible oil cakes with reduced dose of urea on yield and fodder value of Cenchrus setigerus along with gene expression of rhizospheric Azospirillum. Maximum yield of 23.75 t/ha was obtained when 75 % of nitrogen required by the grass was provided by Neem cake and rest by urea. Fodder value in terms of acid detergent fibre, neutral detergent fibre and crude proteins were also significantly (P<0.05) affected by the treatments. The population of free-living Azospirillum in the rhizopheric soil was directly proportional to the amount of cakes applied in soil. With Urea alone, the nifH and ntrC gene activity was found to downregulated by 1.5 and 4.0 folds respectively but integration of cakes (both Jatropha and neem cake) upregulated the nifH and ntrC gene expression.


Non-edible Oil Cakes; Urea; PCR; ; Nif Genes; Upregulation; Downregulation


Bremner, J.M. 1995. Recent research on problems in the use of urea as a nitrogen fertilizer.Fertilizer Research 42(1-3): 321-329.

Caceres, E.A. 1982. Improved medium for isolation of Azospirillum spp. Applied and Environmental Microbiology 44(4): 990-991.

Carroll, C. and Marcey, D. 2003. The N-terminal receiver domain of nitrogen regulatory protein C,CLU Biology Department.

Doberneir, J.; Alef, K. and Nannipieri, P. 1995. Isolation and identification of aerobic nitrogen fixing bacteria from soil and plants, pages 134-141, In: Methods in Applied Soil Microbiology and Biochemistry. Academic Press, London..

Fani, R.; Bazzicalupo, M.; Damiani, G.; Bianchi, A.; Schipani, C.; Sgara-mella, V. and Polsinelli, M.1989.Cloning of histidine genes of Azospirillum brasilense: organisation of the ABFH gene cluster and nucleotide sequence of the hisB gene. Molecular and General Genetics (216): 224-229.

Holguin, G.; Patten, C.L. and Glick, B.R.1999. Genetics and molecular biology of Azospirillum. Biology and Fertility of Soils 29: 10-23.

Hubbell, D.H.; Gaskins, M.H and Dazzo, F.B.1980.Association of Azospirillum with grass roots. Applied and Environmental Microbiology 39(1): 219-226.

Lovell, C.R.; Piceno, J.M. and Quattro, J.M.2000. Molecular analysis of diazotroph diversity in the rhizosphere of the smooth cordgrass, Spartina alterniflora. Applied and Environmental Microbiology 66: 3814–3822.

Nakhro, N. and Dkhar, M.S. 2010. Impact of organic and inorganic fertilizers on microbial populations and biomass carbon in paddy field soil. Journal of Agronomy 9: 102-110.

Nelson, D.W. and Sommers, L.E. 1973. Determination of total nitrogen in plant material. Agronomy Journal 65: 109-112.

Ogbonna, P.E.; Oluah, S.N.; Jidere, C. and Okafor, F.C. 2012. Effects of de-oiled palm kernel cake based fertilizers on sole maize and cassava crops. African Journal of Biotechnology 11(20): 4551-4557.

Pathak, P.S. and Kumar, S. 2004. Forage and grazing resources in different agro-climatic regions of India. Pages 1-42, In: Kundu, S.S., Misra, A.K. and Pathak. P.S. (Editors) Buffalo Production under Different Climatic Regions. International Book Distributing Company, Lucknow.

Schmutterer, H. and Singh, R.P. 1995. List of insect susceptible to Neem product. In: Schmutterer, H.(Editor). The Neem Tree Sources of Unique Natural Products for Integrated Pest Management,medicine,industry and Other Purposes. VCH Publishers, New York.

Sharma, S.; Verma, M. and Sharma, A. 2013. Utilization of Non edible oil seed cakes as substrate for growth of Paecilomyces lilacinus and as biopesticide against termites. Waste Biomass Valorization 4: 325-330.

Sharma, S.; Sharma, A.; Gupta, A.; Mishra, S. and Vasudevan, P. 2012. Effect of biofertilizers on fodder crops under rainfed conditions in semiarid area. International Journal of Environ-mental Sciences 1: 87-96.

Singh, M.; Tripathi, A.K. and Klingmuller, W. 1989. Identification of a regulatory nifA type gene and physical mapping of cloned new nif regions of Azospirillum brasilense. Molecular and General Genetics 219(1-2): 235-240.

Ueda, T.; Suga, Y.; Yahiro, N. and Matsuguchi, T.1995. Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences. Journal of Bacteriology 177(5): 1414-1417.

Van Soest, P.J; Robertson, J.B; Lewis, B.A. 1991.Methods of dietary fiber, neutral detergent fiber and non-polysaccharides in relation to animal nutrition. Journal of Diary Science 74: 3583-3597.

Wennberg, M., and Nyman, M. 2004. On the possibility of using high pressure treatment to modify physico-chemical properties of dietary fibre in white cabbage (Brassica oleraceae var. capitata). Innovative Food Science and Emerging Technologies 5: 171−177.

Widmer, F.; Shaffer, B.T.; Porteous, L.A. and Seidler, R.J. 1999. Analysis of nifH gene pool complexity in soil and litter at a Douglas fir forest site in the Oregon Cascade mountain range. Applied and Environmental Microbiology 65: 374–380.

Zehr, J.P.; Mellon, M.T. and Zani, S. 1998. New nitrogen-fixing microorganisms detected in oligotrophic oceans by amplifi-cation of nitrogenase (nifH) genes. Applied and Environmental Microbiology 64(9): 3444 -3450.

Zhang, X.-X. and Rainey, P.B. 2008. Dual Involvement of CbrAB and NtrBC in the Regulation of Histidine Utilization in Pseudomonas fluorescens SBW25. Genetics 178(1): 185-195.

Zhang, Y.; Burris, R.H.; Ludden, P.W. and Roberts, G.P. 1997. Regulation of nitrogen fixation in Azospirillum brasilense. FEMS Microbiology Letters 152(2):195-204.

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